recN [2017-11-16 19:12:22]

DNA repair and genetic recombination

locus: BSU24240

pI: 4.80

mw: 64.31 kDa

protein length: 576 aa

gene length: 1728 bp

function: DNA repair and genetic recombination

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,520,557 → 2,522,287

The protein

Catalyzed reaction/ biological activity

• RecN stimulates polymerizing activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|PnpA] [Pubmed|21859751]

Protein family

• recN family (according to Swiss-Prot)

Structure

• [PDB|4AD8] (from Deinococcus radiodurans, 30% identity)

[SW|Localization]

• nucleoid (multiple) [Pubmed|16479537]
• evenly distributed in growing cells [Pubmed|21859751]
• upon DNA damage, RecN forms a single focus per nucleoid [Pubmed|21859751], recruitment is affected by the presence of [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|fumarase] [pubmed|29140245]

Expression and Regulation

Operons

• genes: [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
  description: [Pubmed|2106508]

  regulation

  • by [protein|search|sRNA] [protein|search|sr1]

  additional information

  • expression is fourfold increased upon depletion of ''[SW|nusA]'' [http://www.nature.com/articles/nmicrobiol20157 Reference]

• genes: [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Mutant

• 1A891 ( ''recN''::''cat''), [Pubmed|8510642], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A891&Search=1A891 BGSC]
• BKE24240 (Δ[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACAGCATTACACCTCTT, downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA
• BKK24240 (Δ[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACAGCATTACACCTCTT, downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA

Labs working on this gene/protein

• [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

References

Reviews

• 19308706,21517913,22933559,23380520

Original publications

• 15849320,19060143,16385024,17999999,2106508,19730681,16479537,21859751,24373815,15186413,8510642,29140245